PCR
Master mix
qPCR
qRT-PCR
RT
Nucleotides
DNA marker
Purification
Modified enzyme
Protein detection |
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Real Time PCR
Zymeset qPCR Mixes are optimized ready-to-use solutions for real-time quantitative PCR assays with all the components necessary to perform qPCR. Zymeset has mixes for both - probe based approach and intercalating dye approach . Please find suitable mix for your qPCR platform from the table or product list below.
2x qPCR mix is designed for quantitative real-time analysis of DNA samples.
ĦE Strong signals
and high sensitivity due to fluorescent dye.
ĦE High specificity - no primer dimers, no NTC signal.
ĦE Optimized 2x qPCR Master mixes for different real-time PCR instruments.
2x qPCR Master mix is ideally suited for:
ĦE Gene expression analysis
ĦE Microarray validation
ĦE Viral load determination qRT-PCR
Components
2x qPCR mix is a 2X mixture of dNTPs, Hotstart Taq DNA polymerase,MgCl2,
fluorescent detection dye, reference dye (optional), and buffer components.
Comparison quantitative PCR : Zymeset and Brand R
Quantitative qPCR for human £]actin gene
qPCR mix formulations are optimized for different machines.
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Probe |
qPCR Instruments |
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BioRad CFX96, Roche LightCycler 480
MJ Research Opticon, Opticon 2 and Chromo 4,
Corbett Rotor-gene 600,3000
Eppendorf Realplex 2Product Application alplex 4
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ABI,7000,7300,7700,7900,stepOne Plus, StepOne Eppendorf Realplex 4 |
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ABI7500, Stratagene Mx3000,
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ZymesetBiotek
email: max@zymeset.com
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