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Pfu DNA polymerase
Pfu DNA polymerase is a thermostable enzyme isolated from Pyrococcus furiousus. The enzyme replicates DNA at 75¢XC, catalyzing the polymerisation of nucleotides into duplex DNA in the 5¡¦¡X> 3¡¦direction. Pfu DNA Polymerase possesses 3¡¦ ¡X> 5¡¦ exonuclease (proofreading) activity. Nucleotide misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high fidelity synthesis. Pfu DNA Polymerase generated PCR fragments are blunt ¡V ended.
Product list
Concentration: 5000 units/ml
Reaction buffer (10x): Ammonium buffer with 20 mM MgSO 4
Storage buffer: 50 mM TrisHCl (pH 8,2), 0.1 mM EDTA, 1mM DTT, 0,1% Nonidet P40, 0,1% Tween-20, 50% glycerol.
Storage conditions: -20¢XC
Ordering informatiom
Cat No |
Description |
Pack size |
Certificate |
Price $ |
PF0500 |
Pfu DNA polymerase |
500U |
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PF2500 |
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2500U |
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ZymesetBiotek
email: max@zymeset.com
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