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M-MLV Reverse Transcriptase (RNase H-)
MMLV Reverse Transcriptase, encoded by
Moloney Murine Leukemia Virus (MMLV RT) is an RNA-dependent DNA polymerase that synthesizes
the complementary cDNA first strand
from a single-stranded RNA template to which a
primer has been hybridized. MMLV RT will also
extend primers hybridized to single-stranded
DNA. Second strand cDNA synthesis can be
achieved from some mRNA templates without an additional DNA polymerase. M-MLV RT (H- ) can
synthesized 9.5-kb products, the largest RNA component in the reaction.
However, M-MLV RT synthesized more full-length cDNA regardless
of size.
Unit definition:One unit of activity is the amount of enzyme required to incorporate 1 nmole of dTTP into an acid-insoluble form in 10 minutes at 37¢XC using polyA-oligo(dT) as template and primer.
Concentration: 200 u/£gl
Storage conditions: -20¢XC
Ordering informatiom
Cat No |
Description |
Pack size |
Certificate |
Price $ |
RT0100 |
M-MLV RT ase |
10,000U |
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RT0500 |
M-MLV RTase |
50,000U |
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ZymesetBiotek
email: max@zymeset.com
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